Coding

Part:BBa_K1321361:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)


CBDcenA with linker fused to NiBP driven by LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

Our NiBP came from BBa_K1151001.

Our LacI promotor came from BBa_J04500.

References